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KMID : 0358920080350010030
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Journal of the Korean Academy of Pedodontics
2008 Volume.35 No. 1 p.30 ~ p.38
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Gene Expression of Exposure to Mineral Trioxide Aggregate(MTA) on Dental Pulp Cells
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Choi Yoo-Suk
Lee Nan-Young
Lee Sang-Ho
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Abstract
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Dental pulp cells are assumed to possess the capacity to elaborate both bone and dentin matrix under the pathological conditions following tooth injury. The purpose of this study is to examine the effects of mineral trioxide aggregate (MTA) on various gene expression regarding dentinogenesis and cell viability assay in cultured primary human dental pulp cells. The author also examined the effects of this material on cellular alkaline phosphatase activity as a potential indicator of dentinogenesis. For gene expression on MTA, reverse transcriptase polymerase chain reaction was performed using primer sets of glyceraldehyde-3-phosphate dehydrogenase, type I collagen, alkaline phosphatase(ALP), osteonectin, and dentin sialoprotein after 2 and 4 days. Cell viability assay showed that the proportion of MTA-treated pulp cells which had been exposed for 5 days to MTA was higher than that of the control cells. Among the genes investigated in this study, ALP and osteonectin(SPARC) were increased in MTA treated group than in control. These findings suggest that this dental pulp culture system may be useful in the future as a model for studying the mechanisms underlying dentin regeneration after the treatment with MTA. Exposure to MTA material would not induce cytotoxic response in the dental pulp cells. In addition, MTA could influence the behavior of human pulp cells by increasing the ALP activity and SPARC synthesis.
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KEYWORD
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Mineral trioxide aggregate(MTA), Dentinogenesis, Alkaline phosphatase activity, Osteonectin(SPARC)
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